Native analysis does not provide accurate molecular weight information. The gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. Inexpensive Laemmli buffer system, low running costs.Protein transfer in as little as 15 min.The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/Glycine running buffers. 1.The Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. Bio-Rad Gel Electrophoresis SystemsĬriterion and Criterion™ Dodeca™ cells use integral cassettes for gel hand casting. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module. Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. See the electrophoresis system selection guide for more details. Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. The Quick Start Guide to Stain-Free Imaging provides step-by-step instructions on how to obtain stain-free gel images from TGX Stain-Free gels in less than 5 minutes using Bio-Rad’s ImageLab™ software and stain-free enabled imagers. View the V3 Western Workflow video to learn more. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment - from running gels to quantifying proteins. Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.ĭownload the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.īio-Rad's V3 Western Workflow™ is a five-step approach to streamlining your western blotting protocol. More Information Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. 1-D polyacrylamide gel electrophoresis for separation of proteins.Ability to monitor the success of your electrophoresis and transfer steps without additional stainingĪpplications and Uses of TGX Stain-Free FastCast Acrylamide Solutions. Efficient protein transfers in as little as 3 min using the Trans-Blot ® Turbo™ transfer system.Electrophoresis run times as short as 20 min using the Mini-PROTEAN ® Tetra or Criterion™ cells.Stain-free visualization of proteins in your gel or blot in less than 5 min with stain-free enabled imaging systems ( Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ MP, ChemiDoc XRS+, and ChemiDoc Touch Systems).Long shelf life - handcast gels remain fresh for up to 4 weeks at 4☌.Handcast gels ready to run in as little as 30 min.Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions Protein samples can be separated in 20 minutes and then visualized in 5 minutes. TGX Stain-Free chemistry enables rapid fluorescent detection of proteins directly within gels and blots with Bio-Rad's stain-free enabled imagers. TGX Stain-Free FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems. TGX (Tris-Glycine eXtended) formulation retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. TGX Stain-Free FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels.
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